![]() Injection of these ES lines into C57Bl/6 blastocysts will generate chimeras, which should be judged according to the following guidelines: The ES cell line R1, derived from cross of 129/Sv x 129/Sv-Cp mouse strains and other ES cell lines such as D3, W4, AB2.2, AK7.1 derived from different substrains of 129 mouse strain have the agouti coat color. Percentage of agouti coat color varies from 5% to 70%. Grade C chimeras exhibit mostly black coat color with patches of agouti coat color. Some grade B chimeras can be 100% agouti. Black coat color (contributed by embryo donor) should comprise no more than 30%. Grade B chimeras exhibit mostly agouti coat color with some patches of chinchilla (1%-15 %). Small percentage of black coat color contributed by embryo donor may be present (about 20%). Variations of chinchilla can include slate or light gray coat color. Grade A chimeras can exhibit about15% of chinchilla coat or be completely chinchilla. Grade A chimera coat color comprises of significant percentage of agouti (about 70%) with patches of chinchilla coat color. Injection of these ES cells lines into C57Bl/6 will generate tri-colored chimeras, which should be judged according to the following guidelines: Genotype of 129/Ola mouse strain is cch/cch, p/p, Aw/Aw (agouti chinchilla coat color). The ES cell lines derived from 129/Ola mouse strain are named E14, E14.1, E14tg2a, CGR8.8. Guidelines for chimeric coat color estimationĮS cell lines are derived from different mouse strains therefore, chimeras generated by blastocysts injections will have different coat colors.
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